How much do you know about the selection of monoclonal antibodies? -Huaqiang Electronic Network

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How much do you know about the selection of monoclonal antibodies? In the pre-sales and after-sales problems handled by our company, we found that the customer is very entangled in the selection of antibodies. I don’t know if I should choose monoclonal antibodies or polyclonal antibodies. I don’t know how to judge the two antibodies. More practical. In this regard, Xiaobian, with the help of the technical department, summed up several small cockroaches that use monoclonal antibodies to help you experiment and choose. Welcome to read!
First we should understand the choice of monoclonal and polyclonal antibodies. A specific antibody produced by a clone is called a monoclonal antibody. Monoclonal antibodies can be specifically targeted to a single specific antigenic determinant, just as a missile accurately hits a target. On the other hand, even with the same antigenic determinant, antibodies can be produced by several clones in the body, forming several monoclonal antibody hybrids called polyclonal antibodies. In the antigen-antibody reaction, the general monoclonal antibody is specific, but the affinity is relatively small, and the sensitivity of the detection antigen is relatively low; while the specificity of the polyclonal antibody is slightly weak, but the affinity of the antibody is strong, the sensitivity is high, but non-specific staining is prone to occur. (can be avoided by blocking etc.).
Second is the choice of application scope. Some primary antibodies can only be used for Western blotting, or immunohistochemistry, immunofluorescence, immunoprecipitation, etc.; even paraffin sections or frozen sections.
Speciesreactivity of species. This is important, indicating that there may be species differences in this antibody, and that this antibody is suitable for detecting antigens in which species.
Finally, the source of the species, generally the rabbit source is mostly polyclonal; while the mouse source is mostly monoclonal, but there are others. Select the appropriate secondary antibody based on this source. As long as you choose the above four elements, it is not far from the success of the antibody experiment. Xiaobian can only help you here. If you need more details, you can consult the website. Thank you for your support and trust in Shanghai Jinma Bio. Welcome to the elisa kit special offer this month.