How is the sample collected during the Elisa kit test? -Huaqiang Electronic Network

Test - lowercase jpg

Kaixin micro test

Test probe P100-M3

Recently, Wang Meng, a student from Shanghai Fudan University, called to inquire: claiming to be the first experiment. I don't know much about the knowledge of many elisa kits. Please call for some help. The first thing I want to know is how to collect the various samples in the Elisa kit test. What issues should I pay attention to?

First of all, I would like to thank Wang for your trust and support to our company. We will provide you with full technical service and free agent testing service. You can rest assured that you can call us if you don't understand anything. For the questions you asked above, our company gives the following answers, I hope to help you!

First, target sample serum: avoid any cellular irritation during the procedure. Use tubes without pyrogens and endotoxins. After collecting the blood, the red blood cells were quickly and carefully separated by centrifugation at 1000 x g for 10 minutes.

Followed by plasma: EDTA, citrate, heparin plasma can be used for detection. The pellet was removed by centrifugation at 1000 x g for 30 minutes.

Of course, the cell supernatant and the polymer were removed by centrifugation at 1000 x g for 10 minutes.

Finally, the tissue homogenate: the tissue is added to the appropriate amount of physiological saline and mashed. Centrifuge at 1000 xg for 10 minutes and take the supernatant. The above four samples are the most common, you can understand, if there are other samples, you can call us. We provide you with some basic information for sample collection and preservation. You have already sent your email address, you can check it out. Summer high temperature weather samples are not easy to use and should be preserved. Hope to see you next time. Thank you